<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">UEHARA, FUMINARI</style></author><author><style face="normal" font="default" size="100%">TOME, YASUNORI</style></author><author><style face="normal" font="default" size="100%">YANO, SHUYA</style></author><author><style face="normal" font="default" size="100%">MIWA, SHINJI</style></author><author><style face="normal" font="default" size="100%">MII, SUMIYUKI</style></author><author><style face="normal" font="default" size="100%">HIROSHIMA, YUKIHIKO</style></author><author><style face="normal" font="default" size="100%">BOUVET, MICHAEL</style></author><author><style face="normal" font="default" size="100%">MAEHARA, HIROKI</style></author><author><style face="normal" font="default" size="100%">KANAYA, FUMINORI</style></author><author><style face="normal" font="default" size="100%">HOFFMAN, ROBERT M.</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">A Color-coded Imaging Model of the Interaction of α&lt;sub&gt;v&lt;/sub&gt; Integrin-GFP Expressed in Osteosarcoma Cells and RFP Expressing Blood Vessels in Gelfoam® Vascularized &lt;em&gt;In Vivo&lt;/em&gt;</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013-04-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">1361-1366</style></pages><volume><style face="normal" font="default" size="100%">33</style></volume><issue><style face="normal" font="default" size="100%">4</style></issue><abstract><style  face="normal" font="default" size="100%">The integrin family of proteins has been shown to be involved in the malignant behavior of cells. We report here development of a color-coded imaging model that can visualize the interaction between αv integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam® vascularized after implantation in red fluorescent protein (RFP) transgenic nude mice. Human 143B osteosarcoma cells expressing αv integrin-GFP were generated by transfection with an αv integrin-GFP vector. Gelfoam® (5×5 mm) was transplanted subcutaneously in transgenic RFP nude mice. The implanted Gelfoam® became highly vascularized with RFP vessels within 14 days. Skin flaps were made at days 7, 14, 21, 28 after transplantation of Gelfoam® for observing vascularization of the Gelfoam® using fluorescence imaging. Gelfoam® is a useful tool to observe angiogenesis in vivo. 143B cells (5×105) expressing αv integrin-GFP were injected into the Gelfoam® seven days after transplantation of Gelfoam®. Seven days after cancer-cell injection, cancer cells and blood vessels were observed in the Gelfoam® by color-coded confocal microscopy via the skin flap. The 143B cells expressing αv integrin-GFP proliferated into the Gelfoam®, which contained RFP-expressing blood vessels. Strong expression of αv integrin-GFP in 143B cells was observed near RFP vessels in the Gelfoam®. The observation of the behavior of αv integrin-GFP and blood vessels will allow further understanding of the role of αv integrin in cancer cells.</style></abstract></record></records></xml>