RT Journal Article
SR Electronic
T1 An Evaluation of Focal Adhesion Kinase in Breast Cancer by Tissue Microarrays
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1169
OP 1173
VO 33
IS 3
A1 SHYR-MING SHEEN-CHEN
A1 CHUN-YING HUANG
A1 YI-CHIA CHAN
A1 CHING-HUA TSAI
A1 SHUN-YU CHI
A1 SHIH-CHUNG WU
A1 CHAO-CHENG HUANG
A1 REI-PING TANG
A1 HOCK-LIEW ENG
A1 SHEUNG-FAT KO
A1 YI-WEI LEE
A1 JIUN-LUNG LIANG
A1 ENG-YEN HUANG
A1 HUI-CHUN CHEN
A1 KUNG-MING RAU
YR 2013
UL http://ar.iiarjournals.org/content/33/3/1169.abstract
AB Background: Many studies have shown that focal adhesion kinase (FAK) is a positive regulator of tumor progression and invasion. However, there is still very limited information about the role of FAK in breast cancer. Tissue microarrays (TMA) can analyze thousands of tissue samples in a parallel fashion with minimal damage to the origin block. This study was designed with the application of TMA to analyze the FAK status in breast cancer. Patients and Methods: Archival tissue specimens from 98 patients with primary invasive breast cancer were selected and FAK expression was analyzed by immunohistochemical staining with TMA. The data of primary tumor staging, age, estrogen receptor status, lymph node status, histological grading and TNM staging were also collected. Results: There were four patients (4.0%) with grade 1 expression in FAK, 41 patients (41.8%) with grade 2 expression in FAK and 53 patients (54.2%) with grade 3 expression in FAK. There was no significant relationship between FAK expression and age, estrogen receptor status, histological grading, primary tumor staging, lymph node status and TNM stage. By multivariate analysis, the TNM stage was found to be significantly related to the overall five-year survival rate (p<0.00001). Conclusion: Immunohistochemical staining with TMA is a convenient and feasible method. Unfortunately, our preliminary results fail to show meaningful prognostic value of FAK in breast cancer. A larger prospective study is warranted for further evaluation.