RT Journal Article SR Electronic T1 (−)-Gossypol-enriched Cottonseed Oil Inhibits Proliferation and Adipogenesis of Human Breast Pre-adipocytes JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 949 OP 955 VO 33 IS 3 A1 SAIYI ZHONG A1 JOHN LEONG A1 WEIPING YE A1 PINGPING XU A1 SHU-HONG LIN A1 JIE-YU LIU A1 YOUNG C. LIN YR 2013 UL http://ar.iiarjournals.org/content/33/3/949.abstract AB Background: Breast cancer is the most commonly diagnosed cancer in women. Obesity is an important risk factor for developing breast cancer and is one of few risk factors that women can modify to prevent cancer. (−)-Gossypol-enriched cottonseed oil [(−)-GPCSO] contains 65% (−)-gossypol and 35% (+)-gossypol. Previous studies have demonstrated that both (−)-gossypol and (−)-GPCSO have potent anticancer activity against multiple types of cancer, including breast cancer. In addition, (−)-GPCSO reduced body weight gain and food intake in young female rats. However, the role of (−)-GPCSO on adipogenesis in human breast pre-adipocytes remains unclear. Materials and Methods: Primary human breast pre-adipocytes were induced to differentiate in adipogenic medium in the presence of (−)-GPCSO. The proliferation of pre-adipocytes was determined with a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2-H-tetrazolium (MTS) assay. Lipid accumulation and glycerol 3-phosphate dehydrogenase (GPDH) activity were measured during adipocyte differentiation. mRNA expression of cyclin-D1, B-cell lymphoma-2 (BCL-2), Peroxisome Proliferator-Activated Receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα) and leptin was analyzed by real-time PCR. Results: (−)-GPCSO inhibited proliferation of pre-adipocytes and down-regulated the expression of cyclin-D1 and BCL-2. (−)-GPCSO also significantly decreased adipogenesis, as determined by inhibition of GPDH activity, triglyceride content (TG), and down-regulation of the expression of PPARγ, C/EBPα and leptin. Conclusion: These findings suggest that (−)-GPCSO has the potential as a food supplement to inhibit adipogenesis, and therefore, reduce obesity.