PT  - JOURNAL ARTICLE
AU  - QIN HAO
AU  - RAYMOND MCKENZIE
AU  - HUACHEN GAN
AU  - HUA TANG
TI  - Protein Kinases D2 and D3 Are Novel Growth Regulators in HCC1806 Triple-negative Breast Cancer Cells
DP  - 2013 Feb 01
TA  - Anticancer Research
PG  - 393--399
VI  - 33
IP  - 2
4099  - http://ar.iiarjournals.org/content/33/2/393.short
4100  - http://ar.iiarjournals.org/content/33/2/393.full
SO  - Anticancer Res2013 Feb 01; 33
AB  - Aim: The role of protein kinase D (PKD) in the context of breast cancer cell biology is not clear. Materials and Methods: The expression of PKD isoforms was assessed in various breast cancer cell lines and PKD isoform-specific siRNAs and selective inhibitors were used to study the role of PKD in breast cancer cell growth. Results: PKD2 and PKD3 were two major isoforms expressed at the highest levels in tumorgenic HCC1806 triple-negative breast cancer cells. Silencing PKD2 or PKD3 significantly inhibited HCC1806 cell proliferation, and PKD3 silencing had a higher inhibitory effect than PKD2 silencing on cell growth and PKD-mediated signaling. HCC1806 breast cancer cells were highly responsive to PKD inhibitors but not to a general protein kinase C (PKC) inhibitor. Conclusion: We have identified PKD2 and PKD3, especially PKD3, as novel cell growth regulators in HCC1806 triple-negative breast cancer cells. Targeting PKD instead of all PKCs effectively inhibited cell proliferation in a number of breast cancer cell lines.