PT  - JOURNAL ARTICLE
AU  - SHUHEI YOSHIDA
AU  - KAZUKO MATSUMOTO
AU  - TOKUZO ARAO
AU  - HIROKAZU TANIGUCHI
AU  - ISAO GOTO
AU  - TOSHIAKI HANAFUSA
AU  - KAZUTO NISHIO
AU  - YASUHIDE YAMADA
TI  - Gene Amplification of Ribosomal Protein S6 Kinase-1 and -2 in Gastric Cancer
DP  - 2013 Feb 01
TA  - Anticancer Research
PG  - 469--475
VI  - 33
IP  - 2
4099  - http://ar.iiarjournals.org/content/33/2/469.short
4100  - http://ar.iiarjournals.org/content/33/2/469.full
SO  - Anticancer Res2013 Feb 01; 33
AB  - Background: The gene amplification of ribosomal protein S6 kinase 1 and 2 (S6K1 and S6K2) and its clinical relevance in gastric cancer remain unclear. Materials and Methods: A comparative genomic hybridization analysis and DNA copy number assay were performed for nine cancer cell lines. The gene amplification of S6K1 and S6K2 were determined using a DNA copy number assay of 213 gastric cancer tissues. Results: S6K1 and S6K2 amplifications were observed in one and three cancer cell lines, respectively. No amplification of S6K1 was detected in the gastric cancer tissues, while S6K2 amplification was observed in 4.7% of the gastric carcinoma tissues. Patients with stage IV gastric cancer whose tumors exhibited amplification had a significantly shorter overall survival. Conclusion: S6K2 amplification was frequently observed in gastric cancer and was related to a poor prognosis. Our findings may provide novel insight into the dysregulation of mammalian target of rapamycin signaling by S6K2 amplification in gastric cancer.