RT Journal Article SR Electronic T1 Regulation of the Proliferation of Colon Cancer Cells by Compounds that Affect Glycolysis, Including 3-Bromopyruvate, 2-Deoxyglucose and Biguanides JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 401 OP 407 VO 33 IS 2 A1 MICHAEL A. LEA A1 MEHREEN S. QURESHI A1 MICHAEL BUXHOEVEDEN A1 NICOLETTE GENGEL A1 JESSICA KLEINSCHMIT A1 CHARLES desBORDES YR 2013 UL http://ar.iiarjournals.org/content/33/2/401.abstract AB In previous studies performed by our group, we observed that 2-deoxyglucose blocked the acidification of the medium used for culture of colon cancer cells caused by incubation with biguanides and it had an additive inhibitory effect on growth. In the present work, we found that 3-bromopyruvate can also prevent the lowering of pH caused by biguanide treatment. 3-Bromopyruvate inhibited colonic cancer cell proliferation, but the effect was not always additive to that of biguanides and an additive effect was more notable in combined treatment with 3-bromopyruvate and 2-deoxyglucose. The induction of alkaline phosphatase activity by butyrate was not consistently affected by combination with other agents that modified glucose metabolism. The drug combinations that were examined inhibited proliferation of wild-type and p53-null cells and affected colonic cancer lines with different growth rates.