RT Journal Article SR Electronic T1 MicroRNA miR-34b/c Enhances Cellular Radiosensitivity of Malignant Pleural Mesothelioma Cells JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4871 OP 4875 VO 32 IS 11 A1 YUHO MAKI A1 HIROAKI ASANO A1 SHINICHI TOYOOKA A1 JUNICHI SOH A1 TAKAFUMI KUBO A1 KUNIAKI KATSUI A1 TSUYOSHI UENO A1 KAZUHIKO SHIEN A1 TAKAYUKI MURAOKA A1 NORIMITSU TANAKA A1 HIROMASA YAMAMOTO A1 KAZUNORI TSUKUDA A1 TAKUMI KISHIMOTO A1 SUSUMU KANAZAWA A1 SHINICHIRO MIYOSHI YR 2012 UL http://ar.iiarjournals.org/content/32/11/4871.abstract AB Background: We previously reported that epigenetic silencing of microRNA-34b/c (miR-34b/c) plays an important role in the pathogenesis of malignant pleural mesothelioma (MPM). We examined the impact of miR-34b/c restoration on the radiosensitivity of MPM cells. Materials and Methods: We established stable miR-34b/c and scramble transfectants of two MPM cell lines, H2052 and H28. We examined these transfectants by clonogenic survival assay, phosphorylated histone H2AX (γH2AX) foci assay, cell-cycle analysis, and western blotting. Results: The clonogenic survival assay revealed that miR-34b/c radiosensitized MPM cells. γH2AX foci assay showed that DNA double-strand break repair was delayed in miR-34b/c transfectants. The proportion of sub-G1 phase cells was increased in miR-34b/c transfectants after irradiation. miR-34b/c inhibited expression of cyclin-D1, cyclin-dependent kinase 4/6, B-cell lymphoma-2 (BCL-2) and increased cleaved poly (ADP-ribose) polymerase (cPARP) and cleaved caspase-3 after irradiation. Conclusion: Our results indicate that miR-34b/c enhances radiosensitivity by promoting radiation-induced apoptosis and suggested that miR-34b/c might be a useful therapeutic molecule to enhance radiotherapy in MPM.