PT  - JOURNAL ARTICLE
AU  - YUHO MAKI
AU  - HIROAKI ASANO
AU  - SHINICHI TOYOOKA
AU  - JUNICHI SOH
AU  - TAKAFUMI KUBO
AU  - KUNIAKI KATSUI
AU  - TSUYOSHI UENO
AU  - KAZUHIKO SHIEN
AU  - TAKAYUKI MURAOKA
AU  - NORIMITSU TANAKA
AU  - HIROMASA YAMAMOTO
AU  - KAZUNORI TSUKUDA
AU  - TAKUMI KISHIMOTO
AU  - SUSUMU KANAZAWA
AU  - SHINICHIRO MIYOSHI
TI  - MicroRNA miR-34b/c Enhances Cellular Radiosensitivity of Malignant Pleural Mesothelioma Cells
DP  - 2012 Nov 01
TA  - Anticancer Research
PG  - 4871--4875
VI  - 32
IP  - 11
4099  - http://ar.iiarjournals.org/content/32/11/4871.short
4100  - http://ar.iiarjournals.org/content/32/11/4871.full
SO  - Anticancer Res2012 Nov 01; 32
AB  - Background: We previously reported that epigenetic silencing of microRNA-34b/c (miR-34b/c) plays an important role in the pathogenesis of malignant pleural mesothelioma (MPM). We examined the impact of miR-34b/c restoration on the radiosensitivity of MPM cells. Materials and Methods: We established stable miR-34b/c and scramble transfectants of two MPM cell lines, H2052 and H28. We examined these transfectants by clonogenic survival assay, phosphorylated histone H2AX (γH2AX) foci assay, cell-cycle analysis, and western blotting. Results: The clonogenic survival assay revealed that miR-34b/c radiosensitized MPM cells. γH2AX foci assay showed that DNA double-strand break repair was delayed in miR-34b/c transfectants. The proportion of sub-G1 phase cells was increased in miR-34b/c transfectants after irradiation. miR-34b/c inhibited expression of cyclin-D1, cyclin-dependent kinase 4/6, B-cell lymphoma-2 (BCL-2) and increased cleaved poly (ADP-ribose) polymerase (cPARP) and cleaved caspase-3 after irradiation. Conclusion: Our results indicate that miR-34b/c enhances radiosensitivity by promoting radiation-induced apoptosis and suggested that miR-34b/c might be a useful therapeutic molecule to enhance radiotherapy in MPM.