RT Journal Article SR Electronic T1 Effects of Structural Modifications of Daunorubicin on In Vitro Antileukemic Activity JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 5271 OP 5277 VO 32 IS 12 A1 STOJAK, MARTA A1 MAZUR, LIDIA A1 OPYDO-CHANEK, MAŁGORZATA A1 ŁUKAWSKA, MAŁGORZATA A1 OSZCZAPOWICZ, IRENA YR 2012 UL http://ar.iiarjournals.org/content/32/12/5271.abstract AB Background/Aim: In the search for new derivatives of anthracycline antibiotics, formamidinodaunorubicins containing in the amidine group either a morpholine moiety (DAUFmor) or a hexamethyleneimine moiety (DAUFhex) were synthesized. The biological effects of daunorubicin (DAU), DAUFmor and DAUFhex were compared. Materials and Methods: The experiments were performed on human acute lymphoblastic leukemia MOLT-4 cells and human acute myeloblastic leukemia ML-1 cells. The research was conducted using the spectrophotometric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and the electronic Beckman-Coulter method. Results: Temporary changes in the leukemia cell viability, size and count were found. The antileukemic activities of the new DAU analogs were weaker than that of daunorubicin. MOLT-4 cells were more sensitive than ML-1 cells to the action of all agents. Among the formamidinodaunorubicins, DAUFmor appeared to be more active in ML-1 cells than DAUFhex, but there were not differences between the analyzed values in MOLT-4 cells. Conclusion: The structural modifications of daunorubicin were responsible for the different antileukemic potentials of the two formamidinodaunorubicins.