PT  - JOURNAL ARTICLE
AU  - MARTA STOJAK
AU  - LIDIA MAZUR
AU  - MAŁGORZATA OPYDO-CHANEK
AU  - MAŁGORZATA ŁUKAWSKA
AU  - IRENA OSZCZAPOWICZ
TI  - Effects of Structural Modifications of Daunorubicin on <em>In Vitro</em> Antileukemic Activity
DP  - 2012 Dec 01
TA  - Anticancer Research
PG  - 5271--5277
VI  - 32
IP  - 12
4099  - http://ar.iiarjournals.org/content/32/12/5271.short
4100  - http://ar.iiarjournals.org/content/32/12/5271.full
SO  - Anticancer Res2012 Dec 01; 32
AB  - Background/Aim: In the search for new derivatives of anthracycline antibiotics, formamidinodaunorubicins containing in the amidine group either a morpholine moiety (DAUFmor) or a hexamethyleneimine moiety (DAUFhex) were synthesized. The biological effects of daunorubicin (DAU), DAUFmor and DAUFhex were compared. Materials and Methods: The experiments were performed on human acute lymphoblastic leukemia MOLT-4 cells and human acute myeloblastic leukemia ML-1 cells. The research was conducted using the spectrophotometric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and the electronic Beckman-Coulter method. Results: Temporary changes in the leukemia cell viability, size and count were found. The antileukemic activities of the new DAU analogs were weaker than that of daunorubicin. MOLT-4 cells were more sensitive than ML-1 cells to the action of all agents. Among the formamidinodaunorubicins, DAUFmor appeared to be more active in ML-1 cells than DAUFhex, but there were not differences between the analyzed values in MOLT-4 cells. Conclusion: The structural modifications of daunorubicin were responsible for the different antileukemic potentials of the two formamidinodaunorubicins.