<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">ZEBISCH, MICHAEL</style></author><author><style face="normal" font="default" size="100%">KÖLBL, ALEXANDRA C.</style></author><author><style face="normal" font="default" size="100%">SCHINDLBECK, CHRISTIAN</style></author><author><style face="normal" font="default" size="100%">NEUGEBAUER, JULIA</style></author><author><style face="normal" font="default" size="100%">HEUBLEIN, SABINE</style></author><author><style face="normal" font="default" size="100%">ILMER, MATTHIAS</style></author><author><style face="normal" font="default" size="100%">RACK, BRIGITTE</style></author><author><style face="normal" font="default" size="100%">FRIESE, KLAUS</style></author><author><style face="normal" font="default" size="100%">JESCHKE, UDO</style></author><author><style face="normal" font="default" size="100%">ANDERGASSEN, ULRICH</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Quantification of Breast Cancer Cells in Peripheral Blood Samples by Real-Time RT-PCR</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012-12-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">5387-5391</style></pages><volume><style face="normal" font="default" size="100%">32</style></volume><issue><style face="normal" font="default" size="100%">12</style></issue><abstract><style  face="normal" font="default" size="100%">Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. RNA was isolated from the samples and transcribed into cDNA. TaqMan real-time PCR for cytokeratins 8, 18 and 19 was carried out and was correlated to that of 18S. Results: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. In the CAMA-1 cell line, the increase was even greater the more cells were added. Conclusion: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. Its benefit will be validated in samples from patients with breast cancer.</style></abstract></record></records></xml>