PT - JOURNAL ARTICLE AU - MALGORZATA STEC AU - JAROSLAW BARAN AU - MONIKA BAJ-KRZYWORZEKA AU - KAZIMIERZ WEGLARCZYK AU - JOLANTA GOZDZIK AU - MACIEJ SIEDLAR AU - MAREK ZEMBALA TI - Chemokine Receptors and Chemokine Production by CD34<sup>+</sup> Stem Cell-derived Monocytes in Response to Cancer Cells DP - 2012 Nov 01 TA - Anticancer Research PG - 4749--4753 VI - 32 IP - 11 4099 - http://ar.iiarjournals.org/content/32/11/4749.short 4100 - http://ar.iiarjournals.org/content/32/11/4749.full SO - Anticancer Res2012 Nov 01; 32 AB - Background: The chemokine-chemokine receptor (CR) network is involved in the regulation of cellular infiltration of tumours. Cancer cells and infiltrating macrophages produce a whole range of chemokines. This study explored the expression of some CR and chemokine production by cord blood stem cell-derived CD34+ monocytes and their novel CD14++CD16+ and CD14+CD16− subsets in response to tumour cells. Material and Methods: CR expression was determined by flow cytometry and their functional activity by migration to chemoattractants. Monocytes were cultured with tumour cells and the chemokine content was assessed in culture supernatants. Results: CD14++CD16+ monocytes exhibited increased expression of chemokine (C-C) receptor (CCR) 1, while CD14+CD16− of CCR2, chemokine (C-X-C) receptor (CXCR) 1, 2 and 4. The increased expression of CCR2 on CD14+CD16− monocytes was associated with their enhanced migration to monocyte chemoattractant protein–1 (CCL2), MCP-3 (CCL7), MCP-2 (CCL8) and MCP-4 (CCL13), while that of CXCR1 and 2 to interleukin 8 (CXCL8), and CXCR4 to stromal cell-derived factor-1 (CXCL12). Tumour cells induced production of macrophage inflammatory protein-1α (CCL3) MIP-1β and regulated on activation normal T-cells expressed and secreted (CCL5) but not CCL2 or CXCL8, monokine induced by gamma interferon (CXCL9), interferon gamma-induced protein 10 (CXCL10). Conclusion: The studied monocyte subsets, in comparison to those from blood, exhibit different expression of CRs and response to the stimuli that occur from tumour cells.