RT Journal Article
SR Electronic
T1 Non-invasive Fluorescent-protein Imaging of Orthotopic Pancreatic-cancer-patient Tumorgraft Progression in Nude Mice
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3063
OP 3067
VO 32
IS 8
A1 ATSUSHI SUETSUGU
A1 MATTHEW KATZ
A1 JASON FLEMING
A1 MARK TRUTY
A1 RYAN THOMAS
A1 SHIGETOYO SAJI
A1 HISATAKA MORIWAKI
A1 MICHAEL BOUVET
A1 ROBERT M. HOFFMAN
YR 2012
UL http://ar.iiarjournals.org/content/32/8/3063.abstract
AB In order to individualize and therefore have more effective treatment for pancreatic cancer, we have developed a multicolor, imageable, orthotopic mouse model for individual patients with pancreatic cancer by passaging their tumors through transgenic nude mice expressing green fluorescent protein (GFP) and red fluorescent protein (RFP). The tumors acquired brightly fluorescent stroma from the transgenic host mice, which was stably associated with the tumors through multiple passages. In the present study, pancreatic cancer patient tumor specimens were initially established in NOD.CB17-Prkdcscid/NcrCrl (NOD/SCID) mice. The tumors were then passaged orthotopically into transgenic nude mice ubiquitously expressing GFP and subsequently to nude mice ubiquitously expressing RFP. The tumors, with very bright GFP and RFP stroma, were then orthotopically passaged to non-transgenic nude mice. It was possible to image the brightly fluorescent tumors non-invasively longitudinally as they progressed in the non-transgenic nude mice. This non-invasive imageable tumorgraft model will be valuable to screen for effective treatment options for individual patients with pancreatic cancer, as well as for the discovery of improved agents for this treatment-resistant disease.