@article {MUSA4271, author = {MUSILIYU A. MUSA and VEERA L.D. BADISA and LEKAN M. LATINWO and TYISE A. PATTERSON and MARCIA A. OWENS}, title = {Coumarin-based Benzopyranone Derivatives Induced Apoptosis in Human Lung (A549) Cancer Cells}, volume = {32}, number = {10}, pages = {4271--4276}, year = {2012}, publisher = {International Institute of Anticancer Research}, abstract = {In the present investigation, we report on the possible underlying mechanism for the cytotoxicity of compounds: 3-(4-(2-(dimethylamino)ethoxy)-phenyl)-7-methoxy-4-phenyl coumarin 5, 3-(4-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-7-methoxy-4-phenylcoumarin 6, and 3-(4-(2-(diethylamino) ethoxy)phenyl)-7-methoxy-4-methylcoumarin 7 in the human lung (A549) cancer cell line, using Ray Biotech{\textquoteright}s Human Apoptosis Arrays and apoptotic protein antibodies. Apoptosis array results showed differential apoptotic proteins expression in the extracts of cells treated with compounds 5-7. Western blotting demonstrated that compound 5 induced apoptosis and caused cell death in the A549 cell line via an increase (up-regulation) in Bax protein expression (pro-apoptotic pathway) and a slight decrease (down-regulation) in Bcl-2 protein expression (anti-apoptotic pathway) after 6 h of treatment. These observations may provide valuable information on the mechanism by which these coumarin-based benzopyranone derivatives induce cytotoxicity in the human lung (A549) cancer cell line.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/32/10/4271}, eprint = {https://ar.iiarjournals.org/content/32/10/4271.full.pdf}, journal = {Anticancer Research} }