@article {MIWA3643, author = {SHINJI MIWA and NAOTOSHI SUGIMOTO and NORIO YAMAMOTO and TOSHIHARU SHIRAI and HIDEJI NISHIDA and KATSUHIRO HAYASHI and HIROAKI KIMURA and AKIHIKO TAKEUCHI and KENTARO IGARASHI and AKIHIRO YACHIE and HIROYUKI TSUCHIYA}, title = {Caffeine Induces Apoptosis of Osteosarcoma Cells by Inhibiting AKT/mTOR/S6K, NF-κB and MAPK Pathways}, volume = {32}, number = {9}, pages = {3643--3649}, year = {2012}, publisher = {International Institute of Anticancer Research}, abstract = {We previously reported that caffeine-assisted chemotherapy improved the treatment of malignant bone and soft tissue tumours such as osteosarcoma. Caffeine affects tumour cells through various pathways, including phosphatase and tensin homolog deleted on chromosome 10 (PTEN), AKT, Bcl-2{\textendash}associated X protein (BAX), caspase-3 and p53, and has therefore been indicated as being useful for the treatment of malignant tumours. Here, the effects of caffeine on the proliferation of HOS osteosarcoma cells were assessed by WST-8 assay, and the effects on the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), mammalian target of rapamycin (mTOR) and mitogen-activated protein kinase (MAPK) pathways were assessed by western blot analyses. Caffeine inhibited proliferation of HOS cells and suppressed NF-κB, AKT, mTOR/S6K and ERK activities. Our results support those from previous studies relating to the use of caffeine in the treatment of osteosarcoma.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/32/9/3643}, eprint = {https://ar.iiarjournals.org/content/32/9/3643.full.pdf}, journal = {Anticancer Research} }