RT Journal Article
SR Electronic
T1 Significant Anti-invasive Activities of α-Mangostin from the Mangosteen Pericarp on two Human Skin Cancer Cell Lines
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3805
OP 3816
VO 32
IS 9
A1 WANG, JING J.
A1 SANDERSON, BARBARA J.S.
A1 ZHANG, WEI
YR 2012
UL http://ar.iiarjournals.org/content/32/9/3805.abstract
AB Aim: This study aimed at investigating the anti-invasive activities of α-mangostin on human melanoma SK-MEL-28 and squamous cell carcinoma A-431 cell lines. Materials and Methods: Cytotoxicity was tested by the crystal violet assay; anti-invasive activity was detected by the wound healing, cell-matrix adhesion, and boyden chamber assays; and gene regulatory effects by qRT-PCR. Treatments were at non-toxic concentrations (0-1.25 μg/ml for A-431 cells and 0-2.5 μg/ml for SK-MEL-28 cells). Results: α-Mangostin inhibited motility, adhesion, migration and invasion. Invasive ability was reduced to 4% and 20% following α-mangostin treatment compared with untreated A-431 and SK-MEL-28 cells, respectively. Inhibition of gene expression of MMP-2, MMP-9, NF-κB, and Akt1 was involved in the anti-invasive activities on A-431 cells. Inhibition of MMP-2, NF-κB and IκBα was involved for SK-MEL-28 cells. Conclusion: α-Mangostin suppressed the metastatic processes of SK-MEL-28 and A-431 cell lines by differentially regulating metastasis-related genes, showing potential as an anti-metastatic agent.