PT  - JOURNAL ARTICLE
AU  - JING J. WANG
AU  - BARBARA J.S. SANDERSON
AU  - WEI ZHANG
TI  - Significant Anti-invasive Activities of α-Mangostin from the Mangosteen Pericarp on two Human Skin Cancer Cell Lines
DP  - 2012 Sep 01
TA  - Anticancer Research
PG  - 3805--3816
VI  - 32
IP  - 9
4099  - http://ar.iiarjournals.org/content/32/9/3805.short
4100  - http://ar.iiarjournals.org/content/32/9/3805.full
SO  - Anticancer Res2012 Sep 01; 32
AB  - Aim: This study aimed at investigating the anti-invasive activities of α-mangostin on human melanoma SK-MEL-28 and squamous cell carcinoma A-431 cell lines. Materials and Methods: Cytotoxicity was tested by the crystal violet assay; anti-invasive activity was detected by the wound healing, cell-matrix adhesion, and boyden chamber assays; and gene regulatory effects by qRT-PCR. Treatments were at non-toxic concentrations (0-1.25 μg/ml for A-431 cells and 0-2.5 μg/ml for SK-MEL-28 cells). Results: α-Mangostin inhibited motility, adhesion, migration and invasion. Invasive ability was reduced to 4% and 20% following α-mangostin treatment compared with untreated A-431 and SK-MEL-28 cells, respectively. Inhibition of gene expression of MMP-2, MMP-9, NF-κB, and Akt1 was involved in the anti-invasive activities on A-431 cells. Inhibition of MMP-2, NF-κB and IκBα was involved for SK-MEL-28 cells. Conclusion: α-Mangostin suppressed the metastatic processes of SK-MEL-28 and A-431 cell lines by differentially regulating metastasis-related genes, showing potential as an anti-metastatic agent.