RT Journal Article SR Electronic T1 Methylation of the DFNA5 Gene is Frequently Detected in Colorectal Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1319 OP 1322 VO 32 IS 4 A1 KAZUAKI YOKOMIZO A1 YOSHIKUNI HARADA A1 KAZUHIRO KIJIMA A1 KAZUKI SHINMURA A1 MAKIKO SAKATA A1 KAZUMA SAKURABA A1 YOUHEI KITAMURA A1 ATSUSHI SHIRAHATA A1 TETSUHIRO GOTO A1 HIROKI MIZUKAMI A1 MITSUO SAITO A1 GAKU KIGAWA A1 HIROSHI NEMOTO A1 KENJI HIBI YR 2012 UL http://ar.iiarjournals.org/content/32/4/1319.abstract AB Background: Recently, the human deafness, autosomal dominant 5 gene, DFNA5, has frequently been detected in cancer tissues. The methylation status of the DFNA5 gene in colorectal cancer was examined and was compared to the clinocopathological findings. Materials and Methods: Eighty-five tumor samples and corresponding normal tissues were obtained from patients with colorectal cancer who underwent surgery at our hospital. The methylation status of the DFNA5 gene in these samples was examined by quantitative methylation-specific PCR (qMSP). Subsequently, the clinicopathological findings were correlated with the methylation status of the DFNA5 gene. Results: DFNA5 gene methylation was found in 29 (34%) out of the 85 colorectal carcinomas, suggesting that it was frequently observed in colorectal cancer. A significant correlation with methylation was observed for lymphatic vessel invasion and TNM stage (p=0.0268 and p=0.0189, respectively). Conclusion: DFNA5 might act as a tumor suppressor gene and DFNA5 gene methylation might play an important role in the development of colorectal cancer. Our data implicate DFNA5 gene methylation as a novel molecular biomarker in colorectal cancer.