PT  - JOURNAL ARTICLE
AU  - ATSUSHI SUETSUGU
AU  - MATTHEW KATZ
AU  - JASON FLEMING
AU  - MARK TRUTY
AU  - RYAN THOMAS
AU  - SHIGETOYO SAJI
AU  - HISATAKA MORIWAKI
AU  - MICHAEL BOUVET
AU  - ROBERT M. HOFFMAN
TI  - Imageable Fluorescent Metastasis Resulting in Transgenic GFP Mice Orthotopically Implanted with Human-patient Primary Pancreatic Cancer Specimens
DP  - 2012 Apr 01
TA  - Anticancer Research
PG  - 1175--1180
VI  - 32
IP  - 4
4099  - http://ar.iiarjournals.org/content/32/4/1175.short
4100  - http://ar.iiarjournals.org/content/32/4/1175.full
SO  - Anticancer Res2012 Apr 01; 32
AB  - Tumors from pancreatic cancer patients were established in NOD/SCID mice immediately after surgery and subsequently passaged orthotopically in transgenic nude mice ubiquitously expressing green fluorescent protein (GFP). The primary patient tumors acquired GFP-expressing stroma. Subsequent liver metastases, and disseminated peritoneal metastases maintained the stroma from the primary tumor, and possibly recruited additional GFP-expressing stroma, resulting in their very bright fluorescence. The GFP-expressing stroma included cancer-associated fibroblasts and tumor-associated macrophages in both the primary and metastatic tumors. This imageable model of metastasis from a patient-tumor is an important advance over patient “tumorgraft” models currently in use, which are implanted subcutaneously, do not metastasize and are not imageable. The new imageable model of patient pancreatic cancer metastasis provides unique opportunities to identify current and novel antimetastatic therapeutics for individual patients.