TY - JOUR T1 - Clinical Application of MAGE A1-6 RT-Nested PCR for Diagnosis of Lung Cancer Invisible by Bronchoscopy JF - Anticancer Research JO - Anticancer Res SP - 163 LP - 167 VL - 32 IS - 1 AU - KYEONG-CHEOL SHIN AU - EUN YOUNG CHOI AU - JIN HONG CHUNG AU - CHANGHO JEON AU - KWAN HO LEE Y1 - 2012/01/01 UR - http://ar.iiarjournals.org/content/32/1/163.abstract N2 - Background: The main goal of this study was to evaluate the diagnostic efficacy of melanoma-associated antigen (MAGE) A1-6 reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) of bronchial washing fluid for the detection of lung cancer invisible by bronchoscopy. Patients and Methods: To determine the expression of MAGE A1-6 gene in 75 lung carcinomas diagnosed by conventional fluoroscopy-guided lung biopsy and 58 cancer-free controls, RT-nested PCR was performed of bronchial washing fluid. MAGE A1-6 RT-nested PCR data was analyzed according to tumor histology, stage, size, and compared with cytological data. Results: MAGE A1-6 RT-nested PCR displayed higher sensitivity (64.0%) than that of conventional cytology (14.7%). There was no significant correlation between MAGE gene expression and histological types or clinical stage. For tumor size, detection rates were 74.0% in tumor smaller than 3 cm and 58.7% in these larger than 3 cm. Conclusions: MAGE A1-6 RT-nested PCR of bronchial washing fluid may be a useful method for diagnosis of peripheral lung cancer in clinical practice. ER -