RT Journal Article SR Electronic T1 Acridine Orange Inhibits Pulmonary Metastasis of Mouse Osteosarcoma JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4163 OP 4168 VO 31 IS 12 A1 HARUHIKO SATONAKA A1 KATSUYUKI KUSUZAKI A1 KOJI AKEDA A1 MASAYA TSUJII A1 TAKAHIRO IINO A1 TAKESHI UEMURA A1 TAKAO MATSUBARA A1 TOMOKI NAKAMURA A1 KUNIHIRO ASANUMA A1 AKIHIKO MATSUMINE A1 AKIHIRO SUDO YR 2011 UL http://ar.iiarjournals.org/content/31/12/4163.abstract AB Although the survival of patients with osteosarcoma has improved following development of chemotherapy and surgery, the presence of pulmonary metastases indicate a poor prognosis. We developed photodynamic and radiodynamic therapies with acridine orange (AO-PDT and AO-RDT) for minimally invasive surgery to treat musculoskeletal sarcomas and reported a good clinical outcome of local control and limb function. We investigated the effect of AO-PDT using flash-wave light (FWL) on pulmonary metastasis of mouse osteosarcoma. In in vitro and in vivo studies, AO alone and AO-PDT significantly inhibited cell invasion and the growth of pulmonary metastases from primary mouse osteosarcoma. AO may have a specific metastasis-inhibitory effect, different from the effect of AO-PDT. The fluorovisualization effect on pulmonary metastases following intravenous AO administration showed that pulmonary metastases localized on the lung surface were recognized as brilliant green lesions. In conclusion, AO-PDT using FWL inhibited cell invasion and pulmonary metastases in mouse osteosarcoma; therefore, this treatment modality might be applicable for treating pulmonary metastasis from malignant musculoskeletal tumors in humans.