PT - JOURNAL ARTICLE AU - HARUHIKO SATONAKA AU - KATSUYUKI KUSUZAKI AU - KOJI AKEDA AU - MASAYA TSUJII AU - TAKAHIRO IINO AU - TAKESHI UEMURA AU - TAKAO MATSUBARA AU - TOMOKI NAKAMURA AU - KUNIHIRO ASANUMA AU - AKIHIKO MATSUMINE AU - AKIHIRO SUDO TI - Acridine Orange Inhibits Pulmonary Metastasis of Mouse Osteosarcoma DP - 2011 Dec 01 TA - Anticancer Research PG - 4163--4168 VI - 31 IP - 12 4099 - http://ar.iiarjournals.org/content/31/12/4163.short 4100 - http://ar.iiarjournals.org/content/31/12/4163.full SO - Anticancer Res2011 Dec 01; 31 AB - Although the survival of patients with osteosarcoma has improved following development of chemotherapy and surgery, the presence of pulmonary metastases indicate a poor prognosis. We developed photodynamic and radiodynamic therapies with acridine orange (AO-PDT and AO-RDT) for minimally invasive surgery to treat musculoskeletal sarcomas and reported a good clinical outcome of local control and limb function. We investigated the effect of AO-PDT using flash-wave light (FWL) on pulmonary metastasis of mouse osteosarcoma. In in vitro and in vivo studies, AO alone and AO-PDT significantly inhibited cell invasion and the growth of pulmonary metastases from primary mouse osteosarcoma. AO may have a specific metastasis-inhibitory effect, different from the effect of AO-PDT. The fluorovisualization effect on pulmonary metastases following intravenous AO administration showed that pulmonary metastases localized on the lung surface were recognized as brilliant green lesions. In conclusion, AO-PDT using FWL inhibited cell invasion and pulmonary metastases in mouse osteosarcoma; therefore, this treatment modality might be applicable for treating pulmonary metastasis from malignant musculoskeletal tumors in humans.