%0 Journal Article %A HARUHIKO SATONAKA %A KATSUYUKI KUSUZAKI %A KOJI AKEDA %A MASAYA TSUJII %A TAKAHIRO IINO %A TAKESHI UEMURA %A TAKAO MATSUBARA %A TOMOKI NAKAMURA %A KUNIHIRO ASANUMA %A AKIHIKO MATSUMINE %A AKIHIRO SUDO %T Acridine Orange Inhibits Pulmonary Metastasis of Mouse Osteosarcoma %D 2011 %J Anticancer Research %P 4163-4168 %V 31 %N 12 %X Although the survival of patients with osteosarcoma has improved following development of chemotherapy and surgery, the presence of pulmonary metastases indicate a poor prognosis. We developed photodynamic and radiodynamic therapies with acridine orange (AO-PDT and AO-RDT) for minimally invasive surgery to treat musculoskeletal sarcomas and reported a good clinical outcome of local control and limb function. We investigated the effect of AO-PDT using flash-wave light (FWL) on pulmonary metastasis of mouse osteosarcoma. In in vitro and in vivo studies, AO alone and AO-PDT significantly inhibited cell invasion and the growth of pulmonary metastases from primary mouse osteosarcoma. AO may have a specific metastasis-inhibitory effect, different from the effect of AO-PDT. The fluorovisualization effect on pulmonary metastases following intravenous AO administration showed that pulmonary metastases localized on the lung surface were recognized as brilliant green lesions. In conclusion, AO-PDT using FWL inhibited cell invasion and pulmonary metastases in mouse osteosarcoma; therefore, this treatment modality might be applicable for treating pulmonary metastasis from malignant musculoskeletal tumors in humans. %U https://ar.iiarjournals.org/content/anticanres/31/12/4163.full.pdf