PT  - JOURNAL ARTICLE
AU  - ERI HIKITA
AU  - MARIKO ARAI
AU  - SACHIKO TANAKA
AU  - KENJI ONDA
AU  - HIROYA UTSUMI
AU  - BO YUAN
AU  - HIROO TOYODA
AU  - TOSHIHIKO HIRANO
TI  - Effects of Inorganic and Organic Arsenic Compounds on Growth and Apoptosis of Human T-Lymphoblastoid Leukemia Cells
DP  - 2011 Dec 01
TA  - Anticancer Research
PG  - 4169--4178
VI  - 31
IP  - 12
4099  - http://ar.iiarjournals.org/content/31/12/4169.short
4100  - http://ar.iiarjournals.org/content/31/12/4169.full
SO  - Anticancer Res2011 Dec 01; 31
AB  - Background/Aim: To investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Materials and Methods: Cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5¬diphenyltetrazolium bromide (MTT) assay. Apoptotic cell morphology was examined by cell staining with Hoechst 33342. Cellular caspase-3/7 activities were measured after arsenic treatment. Results: The inhibitory concentration by 50% (IC50) values of As2O3 towards MOLT-4 and daunorubicin- resistant MOLT-4/DNR cell proliferation were 0.87 and 0.92 μM, while the values for arsenic acid were 69.1 and 116.6 μM, respectively. These arsenic compounds also inhibited mitogen-induced proliferation of human peripheral blood mononuclear cells. Six organic arsenic compounds did not inhibit leukemia cell proliferation. As2O3 and arsenic acid induced apoptotic cell morphology and increased caspase-3/7 activity in the leukemia cells. Ascorbic acid and buthionine sulfoxide enhanced, while N-acetyl-L-cysteine abated, the suppressive effects of inorganic arsenic compounds on leukemia cell proliferation. Conclusion: As2O3 and arsenic acid inhibit proliferation and induce apoptosis in MOLT-4 and daunorubicine-resistant MOLT-4/DNR cells via glutathione-depletion and subsequent caspase-3/7 activation. Organic arsenic compounds have no inhibitory activity on the leukemia cell proliferation. Inorganic arsenic compounds are suggested as useful agents for treatment of T-lymphoblastoid leukemia.