RT Journal Article
SR Electronic
T1 The Novel Vitamin D Analog ZK191784 Inhibits Prostate Cancer Cell Invasion
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4091
OP 4098
VO 31
IS 12
A1 MARIA STIO
A1 MARIA MARTINESI
A1 ANTONELLA SIMONI
A1 ULRICH ZUEGEL
A1 ANDREAS STEINMEYER
A1 RAFFAELLA SANTI
A1 CRISTINA TREVES
A1 GABRIELLA NESI
YR 2011
UL http://ar.iiarjournals.org/content/31/12/4091.abstract
AB Background: Low serum levels of 1,25(OH)2D3 (1,25D), have been associated with aggressive biologic behavior of prostate cancer (PCa). In the present study, we examined the effects of 1,25D and its novel, low-calcemic analog ZK191784 (ZK) on matrix metalloproteinases (MMPs), as well as on intercellular adhesion molecule-1 (ICAM-1) protein levels in human PCa cell lines LNCaP and DU-145. Materials and Methods: Cells were incubated with either vehicle (control), 1,25D or ZK. MMP-2 and MMP-9 activity was determined by gelatin zymography, while ICAM-1 levels were assessed by Western blot analysis and immunocytochemistry. Results: Compared to the controls, 1,25D and ZK caused a marked dose-dependent decrease in the gelatinolytic activity of the MMPs under study, particularly when ZK was used. Likewise, ICAM-1 was down-regulated in the cells incubated with 1,25D or ZK. Conclusion: Vitamin D analogs appear to be involved in the regulation of extracellular MMP activity and membrane adhesion molecule expression. Further studies, both in vitro and in vivo, are needed to define their role as potential therapeutic tools.