TY - JOUR T1 - A<sub>3</sub> Adenosine Receptor Antagonist, Truncated Thio-Cl-IB-MECA, Induces Apoptosis in T24 Human Bladder Cancer Cells JF - Anticancer Research JO - Anticancer Res SP - 2823 LP - 2830 VL - 30 IS - 7 AU - HEEJONG KIM AU - JEONG WOO KANG AU - SOJUNG LEE AU - WON JUN CHOI AU - LAK SHIN JEONG AU - YOUNG YANG AU - JIN TAE HONG AU - DO YOUNG YOON Y1 - 2010/07/01 UR - http://ar.iiarjournals.org/content/30/7/2823.abstract N2 - Background: Human A3 adenosine receptor (A3AR) plays an essential role in several physiopathological processes. Thus far, A3AR-selective ligands have been evaluated as anti-inflammation and anticancer therapeutic agents. Among these ligands, truncated thio-Cl-IB-MECA is a newly reported antagonist, and its function has not been studied. Materials and Methods: Cell viability was measured by MTS assay. Cell cycle progression was analysed by propidium iodide (PI) flow cytometric assay. The apoptotic effects were investigated by Hoechst staining and annexin V-FITC/PI staining. The signal-transduction mechanism was explored by Western blot. Results: Truncated thio-Cl-IB-MECA induced the growth arrest of T24 cells at sub-G1 phase and provoked apoptosis but not necrosis. Apoptotic death was mediated by the activation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). Conclusion: Since truncated thio-Cl-IB-MECA induces anti-proliferation and apoptotic effects via ERK and JNK activation, it may function as an anticancer agent in human bladder cancer cells. ER -