PT  - JOURNAL ARTICLE
AU  - M. PESTA
AU  - J. KLECKA
AU  - V. KULDA
AU  - O. TOPOLCAN
AU  - M. HORA
AU  - V. ERET
AU  - M. LUDVIKOVA
AU  - M. BABJUK
AU  - K. NOVAK
AU  - J. STOLZ
AU  - L. HOLUBEC
TI  - Importance of miR-20a Expression in Prostate Cancer Tissue
DP  - 2010 Sep 01
TA  - Anticancer Research
PG  - 3579--3583
VI  - 30
IP  - 9
4099  - http://ar.iiarjournals.org/content/30/9/3579.short
4100  - http://ar.iiarjournals.org/content/30/9/3579.full
SO  - Anticancer Res2010 Sep 01; 30
AB  - Background: MicroRNAs (miRNAs), which are endogenously expressed regulatory noncoding RNAs, have an altered expression in tumor tissues. MiRNAs regulate cancer-related processes such as cell growth and tissue differentiation, and therefore, might function as oncogenes or tumor-suppressor genes. The aim of our study was to assess the expression of mir-20a, let-7a, miR-15a and miR-16 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) tissue and to investigate the relation between the expression of miRNAs and the clinicopathological features of PCa. Patients and Methods: The study group comprised 138 patients: 85 patients with BPH and 53 patients with PCa. The total RNA was isolated from the tissue specimen core and miRNA expressions were quantified using a real-time RT-PCR method (TaqMan MicroRNA Assays). U6snRNA was used for the normalization of the miRNA expression. Results: miR-20a expression was significantly higher in the group of patients with a Gleason score of 7-10 in comparison with the group of patients with a Gleason score of 0-6 (p=0.0082). We found no statistical differences in the miRNA expressions (mir-20a, let-7a, miR-15a and miR-16) in the PCa tissue samples in comparison with the BPH tissue samples. Conclusion: Our result shows that the more dedifferentiated PCa cells have a higher expression of miR-20a and this supports the oncogenic role of miR-20a in PCa carcinogenesis. The evaluation of miRNA expression could yield new information about PCa pathogenesis.