@article {GUNTUR3557, author = {VAMSI P. GUNTUR and J. CLIFFORD WALDREP and JENNIFER J. GUO and KIM SELTING and RAJIV DHAND}, title = {Increasing P53 Protein Sensitizes Non-Small Cell Lung Cancer to Paclitaxel and Cisplatin In Vitro}, volume = {30}, number = {9}, pages = {3557--3564}, year = {2010}, publisher = {International Institute of Anticancer Research}, abstract = {Aim: To determine whether increasing p53 protein levels confers enhanced chemosensitivity in non-small cell lung cancer (NSCLC). Materials and Methods: Three NSCLC cell lines, with different endogenous p53 expression, were transfected with wild-type p53 (wt-p53) or CD-1 (truncated wt-p53) genes. Cells were subsequently treated with cisplatin (CDDP) or paclitaxel (PAX). Cell viability was measured using Alamar Blue Assay. Results: Cells transfected with CD-1 expressed 13-38\% higher levels of p53 protein compared to cells transfected with the wt-p53 gene, despite their baseline endogenous levels. CD-1-transfected cells also had higher cell death when treated with CDDP (p\<0.05) or PAX, exhibiting 30-60\% higher death rates than cells transfected with the wt-p53 gene and 130-160\% higher than untransfected cells. A significant positive correlation between p53 protein concentration and cytotoxic response was demonstrated (R2 for CDDP=0.823; R2 for PAX=0.909; p\<0.001). Conclusion: Increasing intracellular p53 protein concentrations can augment the effect of CDDP and PAX in NSCLC, despite the baseline level of p53 protein expression.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/30/9/3557}, eprint = {https://ar.iiarjournals.org/content/30/9/3557.full.pdf}, journal = {Anticancer Research} }