RT Journal Article
SR Electronic
T1 Quercetin Inhibits Human DLD-1 Colon Cancer Cell Growth and Polyamine Biosynthesis
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3501
OP 3507
VO 30
IS 9
A1 MICHELE LINSALATA
A1 ANTONELLA ORLANDO
A1 CATERINA MESSA
A1 MARIA GRAZIA REFOLO
A1 FRANCESCO RUSSO
YR 2010
UL http://ar.iiarjournals.org/content/30/9/3501.abstract
AB Background/Aim: Polyamines and ornithine decarboxylase are involved in cell growth and differentiation. The polyphenol quercetin may exert anti-tumour properties by influencing proliferation, differentiation, and apoptosis. The aim of the study was to investigate the effects of increasing concentrations of quercetin (from 0.1 to 100 μM) on polyamine biosynthesis, cell proliferation, and apoptosis in the DLD-1 cells. Materials and Methods: Polyamine levels and ornithine decarboxylase activity were evaluated by HPLC and radiometric technique, respectively. The proliferative response was estimated by 3-(4,5 dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide (MTT) test and [3H]-thymidine incorporation in cell DNA. Apoptosis was investigated by DNA fragmentation. Results: At concentrations ≥50 μM, quercetin significantly reduced ornithine decarboxylase activity, putrescine and spermidine levels compared to controls and cells treated with 0.1 μM concentration. Quercetin concentrations ≥70 μM caused a significant reduction in the conversion of MTT tetrazolium salt and [3H]-thymidine incorporation. The same concentrations were needed to induce the apoptosis. Conclusion: The present study demonstrates that quercetin can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis. Due to the extensive dietary consumption of polyphenols, such as quercetin, the biological activity of these compounds deserves further investigation.