@article {WILLIAMS3301, author = {R.F. WILLIAMS and T.L. SIMS and L. TRACEY and A.L. MYERS and C.Y.C. NG and H. POPPLETON and A.C. NATHWANI and A.M. DAVIDOFF}, title = {Maturation of Tumor Vasculature by Interferon-β Disrupts the Vascular Niche of Glioma Stem Cells}, volume = {30}, number = {9}, pages = {3301--3308}, year = {2010}, publisher = {International Institute of Anticancer Research}, abstract = {Background: The vascular niche necessary for cancer stem cell maintenance is a potential target for cancer therapy. Materials and Methods: Human glioma xenografts were treated with IFN-β delivered systemically via a liver-targeted, adeno-associated viral vector. The vascular niche was examined with immunofluorescence for glioma stem cells, endothelial cells, and perivascular cells. Results: Although IFN-β was not directly toxic to glioma stem cells in vitro, IFN-β decreased tumor size and the number of stem cells recovered in both heterotopic and orthotopic models. Treatment with IFN-β increased perivascular cells investing the tumor vasculature (6-fold) distancing stem cells from endothelial cells. Additionally, vascular smooth muscle cells co-cultured between stem cells and endothelial cells decreased stem cell recovery. Conclusion: Continuous delivery of IFN-β decreased the number of stem cells in glioma xenografts by disrupting the vascular niche through an increase in perivascular cells, which created a barrier between the glioma stem cells and the endothelial cells.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/30/9/3301}, eprint = {https://ar.iiarjournals.org/content/30/9/3301.full.pdf}, journal = {Anticancer Research} }