PT  - JOURNAL ARTICLE
AU  - LI XIE
AU  - JIA WEI
AU  - XIAOPING QIAN
AU  - GANG CHEN
AU  - LIXIA YU
AU  - YITAO DING
AU  - BAORUI LIU
TI  - CXCR4, A Potential Predictive Marker for Docetaxel Sensitivity in Gastric Cancer
DP  - 2010 Jun 01
TA  - Anticancer Research
PG  - 2209--2216
VI  - 30
IP  - 6
4099  - http://ar.iiarjournals.org/content/30/6/2209.short
4100  - http://ar.iiarjournals.org/content/30/6/2209.full
SO  - Anticancer Res2010 Jun 01; 30
AB  - The aim of this study was to explore predictive genes involved in docetaxel sensitivity of gastric cancer. Materials and Methods: Microarray analysis was performed to explore various gene expression levels between parental and docetaxel-resistant cells. A panel of 11 genes selected according to microarray analysis were validated and tested further in 11 cancer cell lines, resulting in 4 genes, CXCR4, CDK6, USP15 and CDH1. Histoculture drug response assay (HDRA) was used to examine docetaxel sensitivity, while qRT-PCR was used to measure the mRNA levels of the genes in 25 surgically dissected gastric cancer specimens. Results: Only CXCR4 mRNA levels in gastric cancer tissues were correlated with docetaxel sensitivity (R2=0.23, p=0.019) and significantly higher in resistant specimens (p=0.038). AMD3100, a CXCR4 antagonist, enhanced the docetaxel cytotoxicity in vitro. Conclusion: CXCR4 mRNA expression levels may be a potential predictive biomarker in gastric cancer.