RT Journal Article SR Electronic T1 Overexpression of P150, a Part of the Large Subunit of the Eukaryotic Translation Initiation Factor 3, in Colon Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1047 OP 1055 VO 30 IS 4 A1 J. HAYBAECK A1 T. O'CONNOR A1 R. SPILKA A1 G. SPIZZO A1 CH. ENSINGER A1 G. MIKUZ A1 T. BRUNHUBER A1 A. VOGETSEDER A1 I. THEURL A1 W. SALVENMOSER A1 H. DRAXL A1 R. BÄNZIGER A1 F. BACHMANN A1 G. SCHÄFER A1 M. BURGER A1 P. OBRIST YR 2010 UL http://ar.iiarjournals.org/content/30/4/1047.abstract AB Background: P150, a 150 kDa protein, was isolated from virally and oncogene-transformed mouse cell lines, partially purified and cloned. P150 is part of the large subunit of the eukaryotic translation initiation factor 3 with sequence homology to centrosomin A. A significant correlation between p150 expression and malignancy in breast, cervical and esophageal cancer have recently been demonstrated. Materials and Methods: Here, 110 colorectal carcinomas of different grades and stages, including lymph node and liver metastases were compared to adjacent normal mucosa by immunohistochemistry of P150. Western blot analysis of selected cases confirmed the expression levels determined by immunohistochemistry. Additionally, immuno-electron and laser scanning microscopy (LSM) was performed. Results: All investigated carcinomas revealed high levels of p150 protein compared to normal adjacent mucosa. The staining intensity was slightly heterogeneous, and positivity was correlated to the tumor grade with statistically significant differences of p150 expression between normal and neoplastic mucosa (p<0.0001, Kruskal-Wallis test). Western blots confirmed higher expression levels of p150 in the tumor. Immunogold labelling and LSM investigation showed high expression levels of p150 on the rough endoplasmic reticulum and polyribosomes, indicating that p150 is translationally active in these tumors. Conclusion: Thus, we propose that p150 plays an important role in development and growth of colorectal carcinomas. Furthermore, p150 expression might provide us with reliable information on the biological behaviour of tumors and the clinical course of the disease. Copyright© 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved