TY - JOUR T1 - Immunohistochemical Detection of Mutated Epidermal Growth Factor Receptors in Pulmonary Adenocarcinoma JF - Anticancer Research JO - Anticancer Res SP - 5233 LP - 5237 VL - 30 IS - 12 AU - HARUHIKO NAKAMURA AU - ATSUSHI MOCHIZUKI AU - TAKUO SHINMYO AU - KOJI ANDO AU - NORIAKI KURIMOTO AU - KUMIO YOKOTE AU - MASAYUKI TAKAGI Y1 - 2010/12/01 UR - http://ar.iiarjournals.org/content/30/12/5233.abstract N2 - Background: Epidermal growth factor receptor (EGFR) mutational analysis has become essential in determining a therapeutic strategy for lung cancer. This study validated the reliability of recently generated rabbit monoclonal antibodies that recognise specific mutations of the EGFR protein. Patients and Methods: Pulmonary adenocarcinoma tissue from 20 previously genotyped specimens was prepared for immunohistochemical staining by two antibodies that recognise products of in-frame deletions in exon 19 (E746_A750del) and a point mutation that replaces leucine with arginine at codon 858 in exon 21 (L858R) of the EGFR gene. Results: The findings of EGFR-mutation-specific immunohistochemistry were concordant with the results of DNA analysis in 18 of 20 tested samples leading to 90% sensitivity and 100% specificity of the method. Conclusion: This immunohistochemical assay for products of representative EGFR gene mutations may be performed reliably using tissue specimens from resected pulmonary adenocarcinomas. ER -