RT Journal Article
SR Electronic
T1 Detection of Vimentin (<em>VIM</em>) Methylation in the Serum of Colorectal Cancer Patients
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5015
OP 5018
VO 30
IS 12
A1 ATSUSHI SHIRAHATA
A1 KAZUMA SAKURABA
A1 TETSUHIRO GOTO
A1 MITSUO SAITO
A1 KAZUYOSHI ISHIBASHI
A1 GAKU KIGAWA
A1 HIROSHI NEMOTO
A1 KENJI HIBI
YR 2010
UL http://ar.iiarjournals.org/content/30/12/5015.abstract
AB Aim: Detection of colorectal cancer using serum assay of vimentin methylation. Materials and Methods: We attempted to detect vimentin methylation in the serum of colorectal cancer patients using quantitative methylation-specific polymerase chain reaction (qMSP). Results: Of 44 colorectal cancer patients, 4 (9%) exhibited methylation of the vimentin gene in their serum DNA by qMSP. Interestingly, methylation was significantly found in the serum of patients with liver metastasis, peritoneal dissemination, and distant metastasis (p=0.026, p=0.0029 and p=0.0063, respectively), suggesting that vimentin methylation in serum might be detected more frequently in patients with advanced colorectal cancer. Conclusion: The high sensitivity of qMSP makes it possible to detect smaller amounts of tumor DNA in the serum, suggesting that qMSP can be used as a screening method for cancer.