@article {WALSH4861, author = {JARRETT E. WALSH and M. RITA I. YOUNG}, title = {Interrelationship Between Protein Phosphatase 1 and TGF-β in Regulating Motility and Cytoskeletal Architecture of Endothelial Cells}, volume = {30}, number = {12}, pages = {4861--4866}, year = {2010}, publisher = {International Institute of Anticancer Research}, abstract = {Motility of endothelial cells is a requirement for the vascularization of solid malignancies. While tumors have been shown to produce a host of angiogenic factors, including TGF-β, the mechanisms by which such factors regulate endothelial cell motility have not yet been defined. Thus, the role of the serine/threonine phosphatase PP-1 in regulating endothelial cell motility and cytoskeletal architecture was studied. The present study demonstrated that TGF-β stimulation of motility is dependent on PP-1. Likewise, TGF-β was shown to up-regulate paxillin expression through a process that was PP-1 dependent. The interplay between PP-1 and TGF-β was further observed by the induction of cell rounding and the loss of paxillin-actin co precipitations upon PP-1 inhibition and the compensation for these effects by TGF-β. Studies initiated to determine how PP-1 might regulate motility showed its role in maintaining cytoskeletal organization and its capacity to directly dephosphorylate the focal adhesion scaffolding protein paxillin. These studies suggest that the interplay between TGF-β and PP-1 regulates the motility of endothelial cells that is critical to the process of angiogenesis.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/30/12/4861}, eprint = {https://ar.iiarjournals.org/content/30/12/4861.full.pdf}, journal = {Anticancer Research} }