RT Journal Article
SR Electronic
T1 Three-dimensional Cell Culture of Glioma and Morphological Comparison of Four Different Human Cell Lines
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 383
OP 389
VO 30
IS 2
A1 YOSHINOBU MANOME
A1 SHUICHI MIZUNO
A1 NOBUTAKE AKIYAMA
A1 KOUKI FUJIOKA
A1 HIDEKI SAITO
A1 YOSHIAKI HATABA
A1 TOSHIAKI KOBAYASHI
A1 MICHIKO WATANABE
YR 2010
UL http://ar.iiarjournals.org/content/30/2/383.abstract
AB Background: To explore the intracranial behaviors of glioma, a three-dimensional culture was devised and the morphology of four cell lines was examined. Materials and Methods: Bioabsorbable and degradable gelatin was used as the scaffold and T98G, A172, KNS42, and U118MG representative standard malignant glioma cell lines were cultured three-dimensionally. Results: When grown, the cells demonstrated characteristic conformations. The U118MG cells dispersed with numerous fiber formations. In contrast, the KNS42 and A172 cells aggregated, adhering to each other, resulting in the formation of balloon-like structures. The T98G cells demonstrated an intermediate character. Conclusion: The cell lines showed distinct characteristics in three-dimensional culture. This culture method may have a role in elucidating the fundamental character of cells in the human body. Copyright© 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved