RT Journal Article SR Electronic T1 Expression of Vascular Endothelial Growth Inhibitor (VEGI) in Human Urothelial Cancer of the Bladder and its Effects on the Adhesion and Migration of Bladder Cancer Cells In Vitro JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 87 OP 95 VO 30 IS 1 A1 NING ZHANG A1 ANDREW J. SANDERS A1 LIN YE A1 HOWARD G. KYNASTON A1 WEN G. JIANG YR 2010 UL http://ar.iiarjournals.org/content/30/1/87.abstract AB Background: Vascular endothelial growth inhibitor (VEGI) has been implicated in the regulation of tumour-related vasculature in certain solid tumours. However, its role in urothelial tumours is still unclear. In the present study, the role played by VEGI in urothelial tumours of the bladder was investigated. Materials and Methods: The expression of VEGI was examined in cancer human bladder tissues and in a serial of cell lines using immunochemical staining and RT-PCR respectively. The biological impact of modifying VEGI expression in urothelial cancer cells was evaluated using in vitro models. Results: VEGI mRNA was expressed in a wide variety of human cell lines. VEGI expression was seen in normal urothelial and stromal cells, but was found to be reduced or absent in urothelial cancer cells. The positive staining in normal tissue (6/7) was significantly higher than that of urothelial cancer tissues (2/12), p=0.006. Moreover, overexpression of VEGI reduced the motility and adhesion of urothelial cancer cells in vitro. However, the overexpression of VEGI had no bearing on the growth and invasion of urothelial cancer cells. Conclusion: VEGI has an inhibitory effect on cellular motility and adhesion in bladder cancer cells. Taken together with the expression pattern of VEGI in urothelial cancer of the bladder, it suggests that VEGI functions as a negative regulator of aggressiveness during development and progression of bladder cancer. Copyright© 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved