@article {CHAN3043, author = {JASON YONGSHENG CHAN and BENNY KWONG HUAT TAN and SHAO CHIN LEE}, title = {Scutellarin Sensitizes Drug-evoked Colon Cancer Cell Apoptosis through Enhanced Caspase-6 Activation}, volume = {29}, number = {8}, pages = {3043--3047}, year = {2009}, publisher = {International Institute of Anticancer Research}, abstract = {Background: We have reported that resveratrol (RSV) and 5-fluorouracil (5-FU) evoked apoptosis through caspase-6 activation in wild-type (p53+/+) and knockout (p53-/-) HCT116 human colon cancer cells. In this study, we investigated the sensitization effects of scutellarin (SC), a compound isolated from the traditional Chinese herb Erigeron breviscapus, on RSV and 5-FU-evoked apoptosis of these cancer cells. Materials and Methods: The drug-induced apoptosis was qualified by TUNEL staining under fluorescence microscopy, before being quantified by propiodium iodide staining through flow cytometric assay. Results: SC (100 μM) sensitized RSV- (200 μM) and 5-FU (500 μM)-evoked apoptosis in p53+/+ but not p53-/- cells. RSV- and 5-FU-elicited caspase-6 activation was promoted by SC in a time-dependent manner. SC itself did not trigger apoptosis or caspase-6 activation at the concentration tested. Conclusion: SC is a novel sensitizing agent for both RSV- and 5-FU-evoked apoptosis, through the enhancement of caspase-6 activation in a p53-dependent manner.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/29/8/3043}, eprint = {https://ar.iiarjournals.org/content/29/8/3043.full.pdf}, journal = {Anticancer Research} }