TY - JOUR T1 - Effect of Head and Neck Cancer Supernatant and CpG-Oligonucleotides on Migration and IFN-α Production of Plasmacytoid Dendritic Cells JF - Anticancer Research JO - Anticancer Res SP - 3019 LP - 3025 VL - 29 IS - 8 AU - ANNETTE THIEL AU - RALPH PRIES AU - SABRINA JESKE AU - THOMAS TRENKLE AU - BARBARA WOLLENBERG Y1 - 2009/08/01 UR - http://ar.iiarjournals.org/content/29/8/3019.abstract N2 - Background: Plasmacytoid dendritic cells (PDCs) infiltrating solid tumor tissues and draining lymph nodes of head and neck squamous cell carcinoma (HNSCC) show an impaired immune response. Immunosuppressive cytokines secreted by HNSCC have been recognized to account for reduced interferon-α (IFN-α) production, whereas the influence on PDC migration has not yet been investigated. Materials and Methods: PDCs were isolated from human peripheral blood by magnetic bead separation. Cellular functions and characteristics were analyzed using flow cytometry, migration assays and ELISA techniques. Results: We show that HNSCC and CpG oligonucleotides controversially influence the migration and IFN-α production of PDCs. Furthermore we demonstrate that HNSCC induced migration towards stromal cell-derived factor-1 (SDF-1) and macrophage inflammatory protein 1 β (MIP-1β) is not dependent upon the surface density of chemokine (CXC motif) receptor 4 (CXCR4) and chemokine (C-C motif) receptor 5 (CCR5). Conclusion: We propose that HNSCC triggered PDC migration is most likely linked to a multilevel process strongly modulated by tumor microenvironmental influences. ER -