TY - JOUR T1 - Vinorelbine Induces β3-Tubulin Gene Expression through an AP-1 Site JF - Anticancer Research JO - Anticancer Res SP - 3003 LP - 3009 VL - 29 IS - 8 AU - JENNIFER SAUSSEDE-AIM AU - EVA-LAURE MATERA AU - STEPHANIE HERVEAU AU - JEAN-PIERRE ROUAULT AU - CHRISTIANO FERLINI AU - CHARLES DUMONTET Y1 - 2009/08/01 UR - http://ar.iiarjournals.org/content/29/8/3003.abstract N2 - Although the correlation between β3-tubulin expression level in tumors has been well correlated with clinical outcome in patients receiving microtubule-targeted agents, the regulation of this protein remains poorly understood. Recently, new insight of regulatory processes was offered with the cloning of the gene promoter. In this study β3-tubulin gene expression was induced in response to exposure to various antimicrotubule agents such as vinorelbine and paclitaxel. The exploration of the β3-tubulin gene promoter by successive deletions followed by site-directed mutagenesis led to the localization of a vinorelbine-responsive element containing an activator-protein 1 (AP-1) site. Among the various antimicrotubule agents tested, it appeared that the implicated AP-1 site was activated only after exposure to vinorelbine. This study confirms the inducible nature of the β3-tubulin gene promoter. ER -