PT  - JOURNAL ARTICLE
AU  - TERAO, SHUJI
AU  - ACHARYA, BISHNU
AU  - SUZUKI, TORU
AU  - AOI, TAKASHI
AU  - NAOE, MICHIO
AU  - HAMADA, KATSUYUKI
AU  - MIZUGUCHI, HIROYUKI
AU  - GOTOH, AKINOBU
TI  - Improved Gene Transfer into Renal Carcinoma Cells Using Adenovirus Vector Containing RGD Motif
DP  - 2009 Aug 01
TA  - Anticancer Research
PG  - 2997--3001
VI  - 29
IP  - 8
4099  - http://ar.iiarjournals.org/content/29/8/2997.short
4100  - http://ar.iiarjournals.org/content/29/8/2997.full
SO  - Anticancer Res2009 Aug 01; 29
AB  - The transduction efficacy of adenovirus serotype 5 (Ad5) vector in human renal carcinoma cells is generally extremely low due to the non-expression of Coxsackie and adenoviral receptor (CAR). We investigated whether fiber-modified Ad vector containing an RGD motif in the HI loop of the Ad fiber knob could increase the transduction efficiency of Ad5 in human renal carcinoma cells in vitro. Materials and Methods: We examined both expressions of CAR, and αv, β3 and β5 integrins, and the transduction efficacy of fiber-modified adenovirus vector in all cell lines. Results: The expression of CAR was lower and those of αv and β3 integrins were higher in all cell lines compared with control cell line, KK47. The transduction efficacy of fiber-modified Ad vector increased by 125- to 1,800-fold compared with Ad5. Conclusion: The fiber-modified Ad vector may be useful to establish effective new gene therapy strategies for the treatment of renal cell carcinoma.