RT Journal Article
SR Electronic
T1 Antiangiogenic Properties of <em>Viscum Album</em> Extracts Are Associated with Endothelial Cytotoxicity
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2945
OP 2950
VO 29
IS 8
A1 ELLURU, SRI RAMULU
A1 VAN HUYEN, JEAN-PAUL DUONG
A1 DELIGNAT, SANDRINE
A1 PROST, FABIENNE
A1 HEUDES, DIDIER
A1 KAZATCHKINE, MICHEL D.
A1 FRIBOULET, ALAIN
A1 KAVERI, SRINI V.
YR 2009
UL http://ar.iiarjournals.org/content/29/8/2945.abstract
AB Back ground: Viscum album (VA) extracts are widely used in cancer therapy and are known to be cytotoxic to tumors and endothelial cells. Angiogenesis plays an important role in the growth, sustenance and metastasis of tumors. Inhibition of angiogenesis is now being explored as a new therapeutic avenue for cancer. Materials and Methods: The cytotoxicity of VA extracts was analyzed by Annexin V labeling and propidium iodide uptake in EA-hy926 endothelial cells. The antiangiogenic effect was studied in vitro by treating the EA-hy926 cells in matrigel and subsequent analysis of vascular formation. Computer-assisted image analysis of vascular formation was analyzed to quantify the in vitro data. In vivo studies were performed by implanting matrigel ± VA extracts in Balb/C mice that had been subjected to IP treatment with VA extracts. Results: The combination of systemic and intra- matrigel treatment with the VA Qu Spez extract caused significant inhibition of angiogenesis. The VA P extract treatment showed insignificant change in vessel formation. Conclusion: These results may provide novel guidelines towards improved strategies using VA extracts based on the inhibition of angiogenesis of tumors.