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Research ArticleExperimental Studies
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The Effect of Photodynamic Therapy Using 5-Aminolevulinic Acid in Bone and Soft Tissue Sarcoma Cells

RYOHEI ADACHI, TOMOKI NAKAMURA, KENTA NAKATA, TERUYA UCHIYAMA, TOMOHITO HAGI, KUNIHIRO ASANUMA and AKIHIRO SUDO
Anticancer Research March 2024, 44 (3) 963-969; DOI: https://doi.org/10.21873/anticanres.16891
RYOHEI ADACHI
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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TOMOKI NAKAMURA
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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  • For correspondence: tomoki66{at}clin.medic.mie-u.ac.jp
KENTA NAKATA
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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TERUYA UCHIYAMA
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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TOMOHITO HAGI
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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KUNIHIRO ASANUMA
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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AKIHIRO SUDO
Department of Orthopedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan
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    Figure 1.

    The graph shows accumulation of protoporphyrin IX (PpIX) in C2C12 and HT1080 cells after 24 h (A) and 48 h (B) of treatment. The accumulation of PpIX in HT 1080 is significantly higher than that in C2C12 after 24 h (A) and 48 h (B) of treatment (**p<0.01). (Black bar; C2C12, Gray bar; HT1080).

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    Figure 2.

    Western blotting shows weak expression of ferrochelatase (FECH) in sarcoma cell lines compared to that in C2C12 cells.

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    Figure 3.

    The cell proliferation assay showed significant inhibition of cell proliferation in the 100 and 200 μg/ml 5-Aminolevulinic acid (5-ALA) photodynamic therapy (PDT) group groups compared to the group treated with 0 μg/ml 5-ALA PDT at 48 h in 143B (A), LM8 (B), and HT1080 (C) (*p<0.05). (A: 0 μg/ml 5-ALA, B: 10 μg/ml 5-ALA, C: 100 μg/ml 5-ALA, D: 200 μg/ml 5-ALA).

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    Figure 4.

    The 5-Aminolevulinic acid (5-ALA) photodynamic therapy (PDT) group shows a significant inhibition in tumor volume compared with that in the control, 5-ALA alone, and strobe scope light alone groups at 14 days after treatment (A: 143B cells, B: LM8 cells, C: HT1080cells) (*p<0.05, **p<0.01). (A: control, B: 250 mg/kg 5-ALA, C: strobe scope light, D: 5-ALA PDT).

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Anticancer Research: 44 (3)
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Vol. 44, Issue 3
March 2024
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The Effect of Photodynamic Therapy Using 5-Aminolevulinic Acid in Bone and Soft Tissue Sarcoma Cells
RYOHEI ADACHI, TOMOKI NAKAMURA, KENTA NAKATA, TERUYA UCHIYAMA, TOMOHITO HAGI, KUNIHIRO ASANUMA, AKIHIRO SUDO
Anticancer Research Mar 2024, 44 (3) 963-969; DOI: 10.21873/anticanres.16891

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The Effect of Photodynamic Therapy Using 5-Aminolevulinic Acid in Bone and Soft Tissue Sarcoma Cells
RYOHEI ADACHI, TOMOKI NAKAMURA, KENTA NAKATA, TERUYA UCHIYAMA, TOMOHITO HAGI, KUNIHIRO ASANUMA, AKIHIRO SUDO
Anticancer Research Mar 2024, 44 (3) 963-969; DOI: 10.21873/anticanres.16891
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Keywords

  • soft tissue sarcoma
  • osteosarcoma
  • 5-aminolevulinic acid
  • photodynamic therapy
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