Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Anticancer Research
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Anticancer Research

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Subscribers
    • Advertisers
    • Editorial Board
  • Other Publications
    • In Vivo
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
    • 2008 Nobel Laureates
  • About Us
    • General Policy
    • Contact
  • Visit us on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

Streptonigrin Mitigates Lung Cancer-induced Cachexia by Suppressing TCF4/TWIST1-induced PTHLH Expression

XUE-QUAN FANG, SEONGHOON LEE, YOUNG-SEON KIM, GA EUL HAN, CHANG-HOON LIM and JI-HONG LIM
Anticancer Research March 2023, 43 (3) 1149-1157; DOI: https://doi.org/10.21873/anticanres.16260
XUE-QUAN FANG
1Department of Medicinal Biosciences, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea;
2Department of Applied Life Science, Graduate School, BK21 Program, Konkuk University, Chungju, Republic of Korea;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SEONGHOON LEE
1Department of Medicinal Biosciences, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea;
2Department of Applied Life Science, Graduate School, BK21 Program, Konkuk University, Chungju, Republic of Korea;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YOUNG-SEON KIM
2Department of Applied Life Science, Graduate School, BK21 Program, Konkuk University, Chungju, Republic of Korea;
3Jung-Ang Microbe Research Institute (JM), Heungdeok-gu, Cheongju, Republic of Korea;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
GA EUL HAN
1Department of Medicinal Biosciences, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CHANG-HOON LIM
1Department of Medicinal Biosciences, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea;
2Department of Applied Life Science, Graduate School, BK21 Program, Konkuk University, Chungju, Republic of Korea;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
JI-HONG LIM
1Department of Medicinal Biosciences, College of Biomedical & Health Science, Konkuk University, Chungju, Republic of Korea;
2Department of Applied Life Science, Graduate School, BK21 Program, Konkuk University, Chungju, Republic of Korea;
4Center for Metabolic Diseases, Konkuk University, Chungju, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jhlim@kku.ac.kr
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Background/Aim: Cachexia – a wasting disorder of adipose and skeletal muscle tissue – is the most common driver of poor prognosis in patients with advanced lung cancer. Parathyroid hormone-like hormone (PTHLH) is potentially a critical factor in cancer-associated cachexia. We previously showed that streptonigrin – an aminoquinone with antitumor effects – inhibited the interaction between TCF4 and TWIST1. This study aimed to determine the anti-cachectic performance of streptonigrin in lung cancer. Materials and Methods: We assessed the effect of streptonigrin on the interaction of TCF4 and TWIST1 using co-immunoprecipitation and a mammalian-two hybrid luciferase assay, which was confirmed by an in vitro GST pull-down assay using recombinant bHLH domain-containing TCF4 and TWIST1. We assessed the anti-cachectic effect of streptonigrin in vivo using an LLC1 cell-induced tumour-bearing mouse model. Changes in the degree of skeletal muscle and adipose tissue wasting were determined by measuring the weights of gastrocnemius and epidydimal white adipose tissue. Results: Streptonigrin was found to inhibit the interaction of TCF4 with TWIST1 in a dose-dependent manner. The in vitro GST pull-down assay revealed that streptonigrin directly inhibited the interaction between TCF4 and TWIST1. The expression of PTHLH mRNA, which is transcriptionally regulated by the TCF4/TWIST1 complex in response to TGF-β1 signalling, was decreased in streptonigrin-treated lung cancer cells. Streptonigrin significantly decreased the expression of proteolysis-related genes in skeletal muscle and browning-related genes in white adipose tissues of LLC1-induced tumour-bearing mice. Conclusion: Streptonigrin exerts potent therapeutic effects on lung cancer-induced cachexia by suppressing TCF4/TWIST1-mediated PTHLH expression.

Key Words:
  • Lung cancer
  • cachexia
  • TCF4
  • TWIST1
  • PTHLH
  • streptonigrin
  • Received January 20, 2023.
  • Revision received January 31, 2023.
  • Accepted February 1, 2023.
  • Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
View Full Text

This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.

patientACCESS

patientACCESS - Patients desiring access to articles
PreviousNext
Back to top

In this issue

Anticancer Research: 43 (3)
Anticancer Research
Vol. 43, Issue 3
March 2023
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Anticancer Research.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Streptonigrin Mitigates Lung Cancer-induced Cachexia by Suppressing TCF4/TWIST1-induced PTHLH Expression
(Your Name) has sent you a message from Anticancer Research
(Your Name) thought you would like to see the Anticancer Research web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
3 + 1 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Streptonigrin Mitigates Lung Cancer-induced Cachexia by Suppressing TCF4/TWIST1-induced PTHLH Expression
XUE-QUAN FANG, SEONGHOON LEE, YOUNG-SEON KIM, GA EUL HAN, CHANG-HOON LIM, JI-HONG LIM
Anticancer Research Mar 2023, 43 (3) 1149-1157; DOI: 10.21873/anticanres.16260

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Streptonigrin Mitigates Lung Cancer-induced Cachexia by Suppressing TCF4/TWIST1-induced PTHLH Expression
XUE-QUAN FANG, SEONGHOON LEE, YOUNG-SEON KIM, GA EUL HAN, CHANG-HOON LIM, JI-HONG LIM
Anticancer Research Mar 2023, 43 (3) 1149-1157; DOI: 10.21873/anticanres.16260
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • SOX10 Inhibits T Cell Recognition by Inducing Expression of the Immune Checkpoint Molecule PD-L1 in A375 Melanoma Cells
  • The Application of Static Magnetic Stimulation Reduces Survival of SH-SY5Y Neuroblastoma Cells
  • The Prognostic Role of ACO2 in Renal Cell Carcinoma
Show more Experimental Studies

Similar Articles

Keywords

  • Lung cancer
  • cachexia
  • TCF4
  • TWIST1
  • PTHLH
  • streptonigrin
Anticancer Research

© 2023 Anticancer Research

Powered by HighWire