Article Figures & Data
Figures
- Figure 1.
Effect of integrin αvβ8 on oral squamous cell carcinoma cell motility and invasion. A: Western blot analysis of integrin αv and β8 protein expression levels in Ca9-22, KO, SCCKN, and ZA cells. Significantly different from ZA cells at: *p<0.05 and **p<0.01 (Dunnett’s test). B: Modified Boyden chamber assay of SCC cell motility. Results from counting cells in eight optical fields are given as mean±SD of at least three independent experiments. C: Ca9-22, KO, SCCKN, and ZA cell morphology in type I collagen gel. Scale bar=100 μm.
- Figure 2.
Ras homolog family member A (RHOA), Ras-related C3 botulinum toxin substrate 1 (RAC1), and cell division control protein 42 homolog (CDC42) activation in oral squamous cell carcinoma cells on type I collagen gels. Pull-down assays and western blots of GTP activated RHOA (A), CDC42 (B), and RAC1 (C).
- Figure 3.
Effect of Ras-related C3 botulinum toxin substrate 1 (RAC1) inhibition in oral squamous cell carcinoma cell motility and invasion. A: The cells were resuspended in culture medium with different concentrations of RAC1 inhibitor NSC23766 and added to each well of a Chemotaxicell chamber with type I collagen coated filters. After incubation for 24 h at 37°C, the chambers were fixed. Motility Results are represented as percentage of the untreated control, and the mean±SD of triplicate determinations. Significantly different from the untreated control at: *p<0.05 and **p<0.01 (Dunnett’s test). B: Ca9-22 and KO cell morphology in the presence of 10 μM NSC23766 for 12 days in type I collagen gel. Scale bar=100 μm.
- Figure 4.
Suppression of integrin β8 reduces type I collagen-induced motility and activation of Ras-related C3 botulinum toxin substrate 1 (RAC1) in squamous cell carcinoma cells. A: β8 Antisense oligo- or control oligo-transfected Ca9-22 and -KO cells were subsequently added to each Chemotaxicell chamber with type I collagen coated-filters. After incubation for 24 h at 37°C, the cells were fixed and counted. Motility results are represented as percentage of the number of migrating Ca9-22 or KO cells transfected with control oligonucleotide, and the mean±SD of triplicate determinations. **Significantly different at p<0.01 (Student’s t-test). B: Ca9-22 and KO cells transfected with β8 antisense oligo- or control oligo were plated onto type I collagen-coated dishes. The cells were lysed 15 min after plating, and the activation of RAC1 was analyzed by pull-down assay. The band intensity of activated RAC1 was normalized to total RAC1. Error bars indicate SD. **Significantly different at p<0.01 (Student’s t-test). C: Morphology of β8 antisense oligo- and control oligo- transfected Ca9-22 and -KO cells in type I collagen gel. Scale bar=100 μm.
In this issue
Jump to section
Related Articles
Cited By...
- No citing articles found.