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Research ArticleExperimental Studies

Suppression of Inflammatory Cytokine Genes Expression in Vascular Endothelial Cells by Super-low Dose Lipopolysaccharide-activated Macrophages

TERUKO HONDA and HIROYUKI INAGAWA
Anticancer Research August 2022, 42 (8) 4049-4054; DOI: https://doi.org/10.21873/anticanres.15901
TERUKO HONDA
1Department of Medical Technology, School of Life and Environmental Science, Azabu University, Kanagawa, Japan;
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  • For correspondence: hondat@azabu-u.ac.jp
HIROYUKI INAGAWA
2Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan;
3Control of Innate Immunity Collaborative Innovation Partnership, Kagawa, Japan
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    Figure 1.

    IL-8 concentration in low-dose lipopolysaccharide (LPS)-activated THP-1 culture medium. THP-1 culture medium was collected 24 h after LPS-activated medium. The IL-8 concentration in THP-1 culture medium with and without LPS activation was measured.

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    Figure 2.

    Gene expression of IL1B, IL6, IL8, and TNFA in human aortic endothelial cells (HAoEC) through low-dose lipopolysaccharide (LPS)-activated macrophages. The mRNA expression of IL1B, IL6, IL8, and TNFA in HAoEC was analyzed using quantitative real-time PCR. Relative quantification was performed by normalizing the target expression to that of ACTB. *p<0.05.

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    Figure 3.

    Gene expression of TGFB1 in human aortic endothelial cells (HAoEC) through low-dose lipopolysaccharide (LPS)-activated macrophages. The mRNA expression of TGFB1 in human aortic endothelial cells (HAoEC) was analyzed using quantitative real-time PCR. Relative quantification was performed by normalizing the target expression to that of ACTB.

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    Figure 4.

    Gene expression of SERPINE, TF, TM, and t-PA in human aortic endothelial cells (HAoEC) through low-dose lipopolysaccharide (LPS)-activated macrophages. The mRNA expression of SERPINE, TF, TM, and t-PA in HAoEC was analyzed using quantitative real-time PCR. Relative quantification was performed by normalizing the target expression to that of ACTB. *p<0.05.

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Anticancer Research: 42 (8)
Anticancer Research
Vol. 42, Issue 8
August 2022
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Suppression of Inflammatory Cytokine Genes Expression in Vascular Endothelial Cells by Super-low Dose Lipopolysaccharide-activated Macrophages
TERUKO HONDA, HIROYUKI INAGAWA
Anticancer Research Aug 2022, 42 (8) 4049-4054; DOI: 10.21873/anticanres.15901

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Suppression of Inflammatory Cytokine Genes Expression in Vascular Endothelial Cells by Super-low Dose Lipopolysaccharide-activated Macrophages
TERUKO HONDA, HIROYUKI INAGAWA
Anticancer Research Aug 2022, 42 (8) 4049-4054; DOI: 10.21873/anticanres.15901
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Keywords

  • macrophages
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  • vascular endothelial cell
  • inflammatory cytokine gene
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  • lipopolysaccharide
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