Article Figures & Data
Figures
- Figure 1.
Macroscopic findings of each PDX model. (a) Subcutaneous engraftment, (b) pancreatic engraftment, (c) peritoneal engraftment, (d) liver engraftment.
- Figure 2.
Hematoxylin and eosin and immunohistochemical staining of primary (X0) and xenograft tumors (X1, X2, X3). Histological differentiation of cancer cells in X0 was maintained in X1 and X2. Furthermore, the positive staining for p53 observed in X0 was also observed in X1. αSMA staining showed no change in fiber formation in each organ. However, when X2 was passed on to X3, the amount of fiber formation was significantly decreased (p<0.01). Scale bar=200 μm.
- Figure 3.
Immunostaining area in PDX stroma (%). (a) CD31, (b) VEGF-A, (c) aSMA. *p<0.05.
- Figure 4.
Immunohistochemical staining of primary tumor and liver transplantation models. (a) X0 (hCD31; reactive human), (b) liver engraftment PDX (hCD31: reactive human), (c) liver engraftment PDX (rCD31: reactive rat), (d) liver engraftment PDX (VEGF-A).CD31, which is cross reactive in humans in which X0 stromal vessels are stained, did not stain in X1 stromal vessels (b: negative staining). In contrast, X1 stromal vessels are stained with CD31, which is cross reactive in rats (c: positive staining). (arrow: stained vascular endothelial cells). Scale bar=200 μm.
