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Research ArticleExperimental Studies

Micro-RNA378a-3p Induces Apoptosis in Sarcomatoid Renal Cell Carcinoma and Regulates POLR2A and RUNX2 Expression

WEN-HUI WENG, KAI-JIE YU, JU-WEN JHAN, SWO-NENG PANG, YUEH-ER CHIOU and SEE-TONG PANG
Anticancer Research February 2022, 42 (2) 811-825; DOI: https://doi.org/10.21873/anticanres.15539
WEN-HUI WENG
1Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei, Taiwan, R.O.C.;
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  • For correspondence: wendywwh@ntut.edu.tw
KAI-JIE YU
1Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei, Taiwan, R.O.C.;
2Division of Urology, Department of Surgery, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan, R.O.C.;
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JU-WEN JHAN
1Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei, Taiwan, R.O.C.;
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SWO-NENG PANG
3Department of General Medicine, Mater Misericordiae University Hospital, Dublin, Ireland;
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YUEH-ER CHIOU
4Department of Nursing, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan, R.O.C.
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SEE-TONG PANG
2Division of Urology, Department of Surgery, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan, R.O.C.;
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  • For correspondence: pst64lab@gmail.com
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Abstract

Background/Aim: Sarcomatoid renal cell carcinoma (sRCC) is an aggressive subtype of RCC. In the current study, we investigated whether the POLR2A and RUNX2 genes are involved in RCC-related signaling pathway and associated with miR-378. Materials and Methods: Thirteen formalin-fixed and paraffin-embedded RCC samples were collected. Three software programs were used to predict the potential gene regulation in RCC by miR-378 and immunohistochemistry analysis was applied to confirm the expression of targeted proteins in FFPE samples. MicroRNA-378 transfection, analysis of mRNA and protein expression via Western Blotting and cell apoptosis analysis via flow cytometry for POLR2A and RUNX2 were further studied in four renal cell carcinoma cell lines. Results: Both the mRNA and protein expression levels of POLR2A and RUNX2 in sRCC cell lines and were significantly higher than those in other subtypes of RCC, and similar results were obtained in clinical samples (p<0.01). Second, overexpression of miR-378 significantly suppressed the expression of POLR2A and RUNX2 in sRCC cells (p<0.01) and enhanced apoptosis (p<0.05). Conclusion: miR-378 significantly inhibits the expression of POLR2A and RUNX2 in sRCC and further promotes apoptosis of sRCC cells. We speculated that the apoptosis mechanism in sRCC occurs via regulation of the ERK2 and PI3K/AKT pathways, which might distinguish it from other subtypes of RCC.

Key Words:
  • Sarcomatoid renal cell carcinoma
  • POLR2A
  • RUNX2
  • micro-RNA378a-3p
  • ERK2 (MAPK1)
  • Received September 26, 2021.
  • Revision received December 8, 2021.
  • Accepted December 13, 2021.
  • Copyright © 2022 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 42 (2)
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Micro-RNA378a-3p Induces Apoptosis in Sarcomatoid Renal Cell Carcinoma and Regulates POLR2A and RUNX2 Expression
WEN-HUI WENG, KAI-JIE YU, JU-WEN JHAN, SWO-NENG PANG, YUEH-ER CHIOU, SEE-TONG PANG
Anticancer Research Feb 2022, 42 (2) 811-825; DOI: 10.21873/anticanres.15539

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Micro-RNA378a-3p Induces Apoptosis in Sarcomatoid Renal Cell Carcinoma and Regulates POLR2A and RUNX2 Expression
WEN-HUI WENG, KAI-JIE YU, JU-WEN JHAN, SWO-NENG PANG, YUEH-ER CHIOU, SEE-TONG PANG
Anticancer Research Feb 2022, 42 (2) 811-825; DOI: 10.21873/anticanres.15539
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Keywords

  • Sarcomatoid renal cell carcinoma
  • POLR2A
  • RUNX2
  • micro-RNA378a-3p
  • ERK2 (MAPK1)
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