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In Vitro Anticancer Activity of the Light Stable Zinc Isotope (64Zn) Compounds

PETER NOVAK, ALEXANDR BALAKIN and MAX TEMNIK
Anticancer Research December 2022, 42 (12) 5685-5698; DOI: https://doi.org/10.21873/anticanres.16077

Article Figures & Data

Figures

  • Figure 1.
    Figure 1.

    Graphic image of a Zn64 aspartate molecule. Schematic representation (A) and 3D model (B).

  • Figure 2.
    Figure 2.

    Synthesis scheme of Zn64 aspartate from L-aspartic acid and 64Zn oxide enriched stable light isotope to 99.2% mass fraction of total zinc.

  • Figure 3.
    Figure 3.

    Graphic image of a Zn64 glutamate molecule. Schematic representation (A) and 3D model (B).

  • Figure 4.
    Figure 4.

    Synthesis scheme of Zn64 aspartate from L-glutamic acid and 64Zn oxide enriched stable light isotope to 99.2% mass fraction of total zinc.

  • Figure 5.
    Figure 5.

    In vitro cytotoxic activity of light stable 64Zn isotope compounds against human breast cancer cells of the luminal MCF-7 (A) and basal MDA-MB-231 (B) cell line subtypes. *p<0.05 as compared to cells treated with Zn64 sulfate. Zn64 asp, Zn64 aspartate; Zn64 glu, Zn64 glutamate; Zn64 sulf, Zn64 sulfate.

  • Figure 6.
    Figure 6.

    In vitro cytotoxic activity of light stable 64Zn isotope compounds against human lung cancer cells (A-549 cell line). *p<0.05 as compared to cells treated with Zn64 sulfate. Zn64 asp, Zn64 aspartate; Zn64 glu, Zn64 glutamate; Zn64 sulf, Zn64 sulfate.

  • Figure 7.
    Figure 7.

    In vitro cytotoxic activity of light stable 64Zn isotope compounds against human acute leukemia cells (HL-60 cell line). *p<0.05 as compared to cells treated with Zn64 sulfate. Zn64 asp, Zn64 aspartate; Zn64 glu, Zn64 glutamate; Zn64 sulf, Zn64 sulfate.

  • Figure 8.
    Figure 8.

    In vitro cytotoxic activity of light stable 64Zn isotope compounds against murine melanoma cells (MB16 cell line). *p<0.05 as compared to cells treated with Zn64 sulfate. Zn64 asp, Zn64 aspartate; Zn64 glu, Zn64 glutamate; Zn64 sulf, Zn64 sulfate.

  • Figure 9.
    Figure 9.

    In vitro comparative analysis of cytotoxic activity of 64Zn aspartate (Zn64 asp) and Zn aspartate (Zn asp) with natural isotopic distribution against tumor cells of different histogenetic patterns. *p<0.05 as compared to cells the same cell line treated with Zn aspartate with natural isotopic distribution (Zn asp).

  • Figure 10.
    Figure 10.

    In vitro comparative analysis of cytotoxic activity of Zn64 (Zn64 asp) aspartate and Zn aspartate (Zn asp) against normal cells of different histogenetic origins. *p<0.05 as compared to cells the same cell line treated with Zn aspartate with natural isotopic distribution (Zn asp). #p<0.05 as compared to MDBK cells treated with Zn64 aspartate.

  • Figure 11.
    Figure 11.

    Changes in the expression of apoptosis regulatory proteins in A-549 cells after their treatment with Zn64 aspartate (Zn64 asp). The bar chart shows the densitometry results of apoptosis-associated protein bands (A). *p<0.05 as compared to control cells. Western blot images are representative of two independent experiments. Lanes 1-3 correspond to A-549 control (cells + physiological saline) and lanes 4-6 to A-549 + Zn64 aspartate (B).

  • Figure 12.
    Figure 12.

    Changes in the expression of apoptosis regulatory proteins in MB16 cells after their treatment with Zn64 aspartate (Zn64 asp). The bar chart shows the densitometry results of apoptosis-associated protein bands (A). *p<0.05 as compared to control cells. Western blot images are representative of two performed independent experiments. Lanes 1-3 correspond to MB16 control (cells + physiological saline) and lanes 4-6 to MB16 + Zn64 aspartate.

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In Vitro Anticancer Activity of the Light Stable Zinc Isotope (64Zn) Compounds
PETER NOVAK, ALEXANDR BALAKIN, MAX TEMNIK
Anticancer Research Dec 2022, 42 (12) 5685-5698; DOI: 10.21873/anticanres.16077
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