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Research ArticleClinical Studies

Simultaneous Detection and HER2 Profiling of Circulating Breast Cancer Cells in Clinical Patients Using a Rare Cell Sorter

TOMOKO MORI, ATSUKO FURUKAWA, TURAN BILAL, AYA UEDA, HIROKO BANDO, TAISUKE MASUDA, FUMIHITO ARAI and SATOSHI MATSUSAKA
Anticancer Research October 2022, 42 (10) 4879-4886; DOI: https://doi.org/10.21873/anticanres.15993
TOMOKO MORI
1Department of Clinical Research and Regional Innovation, University of Tsukuba, Tsukuba, Japan;
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ATSUKO FURUKAWA
1Department of Clinical Research and Regional Innovation, University of Tsukuba, Tsukuba, Japan;
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TURAN BILAL
2Department of Mechanical Engineering, The University of Tokyo, Tokyo, Japan;
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AYA UEDA
3Department of Breast and Endocrine Surgery, University of Tsukuba, Tsukuba, Japan;
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HIROKO BANDO
3Department of Breast and Endocrine Surgery, University of Tsukuba, Tsukuba, Japan;
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TAISUKE MASUDA
2Department of Mechanical Engineering, The University of Tokyo, Tokyo, Japan;
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FUMIHITO ARAI
2Department of Mechanical Engineering, The University of Tokyo, Tokyo, Japan;
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SATOSHI MATSUSAKA
1Department of Clinical Research and Regional Innovation, University of Tsukuba, Tsukuba, Japan;
4Tsukuba Clinical Research and Development Organization, University of Tsukuba, Tsukuba, Japan
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  • For correspondence: matsusaka-s@md.tsukuba.ac.jp
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Abstract

Background/Aim: This study describes a rare cell sorter (RCS) method to detect circulating tumor cells (CTCs) and CTC clusters in whole blood without pretreatment. Patients and Methods: We collected samples from breast cancer patients at the University of Tsukuba Hospital. A total of 15 whole-blood specimens from patients with breast cancer were collected and analyzed via a microfluidics chip, fluorescence-conjugated antibody staining, and fluorescence microscopy. Of 15 total cases, eight were analyzed by RCS ver3 and seven were analyzed by RCS ver3.5 to reveal potential clinical differences in scanning methods. We then examined the HER2 status on 4 of the 15 patients using our RCS system. Results: RCS efficiently detected all subtypes of CTCs and CTC clusters from the peripheral blood of cancer patients. The concordance rate of HER2 status between tissue and CTCs in 4 tested clinical samples was 100%. Conclusion: RCS is a non-invasive method that allows for simultaneous detection of CTCs, cluster presence, and surface marker (e.g., HER2) status. Frequent sampling is, thus, possible and the large amount of data obtained will be clinically useful to predict response to therapy as well as plan adjunct support therapies in cancer patients.

Key Words:
  • Circulating tumor cells
  • circulating tumor cell cluster
  • breast cancer
  • Received July 19, 2022.
  • Revision received August 10, 2022.
  • Accepted August 24, 2022.
  • Copyright © 2022 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
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Anticancer Research: 42 (10)
Anticancer Research
Vol. 42, Issue 10
October 2022
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Simultaneous Detection and HER2 Profiling of Circulating Breast Cancer Cells in Clinical Patients Using a Rare Cell Sorter
TOMOKO MORI, ATSUKO FURUKAWA, TURAN BILAL, AYA UEDA, HIROKO BANDO, TAISUKE MASUDA, FUMIHITO ARAI, SATOSHI MATSUSAKA
Anticancer Research Oct 2022, 42 (10) 4879-4886; DOI: 10.21873/anticanres.15993

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Simultaneous Detection and HER2 Profiling of Circulating Breast Cancer Cells in Clinical Patients Using a Rare Cell Sorter
TOMOKO MORI, ATSUKO FURUKAWA, TURAN BILAL, AYA UEDA, HIROKO BANDO, TAISUKE MASUDA, FUMIHITO ARAI, SATOSHI MATSUSAKA
Anticancer Research Oct 2022, 42 (10) 4879-4886; DOI: 10.21873/anticanres.15993
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Keywords

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